EDTA (0.5M, pH 8.0)

Add 186.1gm of disodium EDTA.2H2O to 800ml of distilled water. Stir vigorously on a magnetic stirrer. Adjust the pH to 8.0 with NaOH (approx. 20g of NaOH pellets).

Dispense into aliquots and sterilize by autoclaving.

The disodium salt of EDTA will not go into solution until the pH of the solution is adjusted to approx. 8.0 by the addition of NaOH.






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