DNase free RNase

Dissolve pancreatic RNase (RNase A) at a concentration of 10 mg/ ml in 0.01M Sodium acetate (pH 5.2) or in distilled water heat to 1000C for 15 minutes. Allow it to cool slowly room temperature.

Adjust the pH by adding 0.1 volume of Tris-Cl (pH 7.4).

Disperse into aliquots and store at -200C.

RNase precipitates when concentrated solutions are heated to 1000C at neutral pH.