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Rasayan - Preparation of common chemicals
May 19, 2007
Tris EDTA (10X)
pH 7.4
100mM Tris-Cl (pH 7.4)
10mM EDTA (pH 8.0)
pH 7.6
100mM Tris-Cl (pH 7.6)
10mM EDTA (pH 8.0)
pH 8.0
100mM Tris-Cl (pH 8.0)
10mM EDTA (pH 8.0)
Sterilize solutions by autoclaving.
Store the solution at room temperature.
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Contents
Ammonium Acetate (10 M)
Calcium Chloride
EDTA (0.5M, pH 8.0)
Ethidium Bromide solution (10mg/ml)
Gel loading buffer (60X stock)
Lysozym (10mg/ml) stock
TBE Buffer
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