Tris EDTA (10X)

pH 7.4
100mM Tris-Cl (pH 7.4)
10mM EDTA (pH 8.0)

pH 7.6
100mM Tris-Cl (pH 7.6)
10mM EDTA (pH 8.0)

pH 8.0
100mM Tris-Cl (pH 8.0)
10mM EDTA (pH 8.0)

Sterilize solutions by autoclaving.
Store the solution at room temperature.

DNase free RNase

Dissolve pancreatic RNase (RNase A) at a concentration of 10 mg/ ml in 0.01M Sodium acetate (pH 5.2) or in distilled water heat to 1000C for 15 minutes. Allow it to cool slowly room temperature.

Adjust the pH by adding 0.1 volume of Tris-Cl (pH 7.4).

Disperse into aliquots and store at -200C.

RNase precipitates when concentrated solutions are heated to 1000C at neutral pH.

Lysozym (10mg/ml) stock

Dissolve solid lysozyme at a concentration of 10mg/ml in 10mM tris-Cl (pH 8.0) immediately before use. Make sure that the pH of the tris-Cl solution is 8.0 before dissolving the protein.

Lysozyme will not work efficiently if the pH of the solution is less than 8.0.






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