Sodium acetate (3M, pH 5.2 and pH 7.0)

Dissolve 408.3g of sodium acetate.3H2O in 800ml of water. Adjust the pH to 5.2 with glacial acetic acid, or adjust the pH to 7.0 with dilute acetic acid. Adjust the volume to 1 liter with water.

Sterilize by autoclaving.

DNase free RNase

Dissolve pancreatic RNase (RNase A) at a concentration of 10 mg/ ml in 0.01M Sodium acetate (pH 5.2) or in distilled water heat to 1000C for 15 minutes. Allow it to cool slowly room temperature.

Adjust the pH by adding 0.1 volume of Tris-Cl (pH 7.4).

Disperse into aliquots and store at -200C.

RNase precipitates when concentrated solutions are heated to 1000C at neutral pH.