Tris-Cl (1M)

Dissolve 121.1g of tris base in 800ml of distilled water. Adjust the pH to the desired volume by adding concentrated HCl.

pH---- HCl
7.4--- 70ml
7.5--- 60ml
8.0--- 42ml


Allow the solution to cool to room temperature before making final adjustments to the pH.

Adjust the volume of the solution to 1 litre with water. Dispense into aliquots and sterilize by autoclaving.

If the 1M solution has a yellow colour, discord it and obtain tris of better quality.

The pH of tris solution is temperature dependant and decreases approx. 0.03 pH units for each 10C increase in temperature.

Sodium acetate (3M, pH 5.2 and pH 7.0)

Dissolve 408.3g of sodium acetate.3H2O in 800ml of water. Adjust the pH to 5.2 with glacial acetic acid, or adjust the pH to 7.0 with dilute acetic acid. Adjust the volume to 1 liter with water.

Sterilize by autoclaving.

NaOH (10N)

The preparation of 10N NaOH involves a highly exothermic reaction, which can cause breakage of glass contain. Prepare this solution with extreme care in plastic beakers.

To 800ml of distilled water, slowely add 400g of NaOH pellets, stirring continuously.

As an added precaution, place the beaker on ice. When the pellets have dissolved completely adjust the volume to 1 litre with water. Store the solution in a plastic container at room temperature.

Sterilization is not necessary.