Tris-Cl (1M)

Dissolve 121.1g of tris base in 800ml of distilled water. Adjust the pH to the desired volume by adding concentrated HCl.

pH---- HCl
7.4--- 70ml
7.5--- 60ml
8.0--- 42ml


Allow the solution to cool to room temperature before making final adjustments to the pH.

Adjust the volume of the solution to 1 litre with water. Dispense into aliquots and sterilize by autoclaving.

If the 1M solution has a yellow colour, discord it and obtain tris of better quality.

The pH of tris solution is temperature dependant and decreases approx. 0.03 pH units for each 10C increase in temperature.